THE DEFINITIVE GUIDE TO ARV-471

The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products and solutions were digested and cloned right into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A degree mutation was launched by the quick modify web site-directed mutagene